Welcome to Li's group!

Publications

"Designing redox potential-controlled protein switches based on mutually exclusive proteins". Qing Peng, Na Kong, Hui-Chuan Eileen Wang, and Hongbin Li. Protein Science, 21, 1222-1230, (2012).

"Single Molecule Force Spectroscopy Reveals Critical Roles of Hydrophobic Core Packing in Determining the Mechanical Stability of Protein GB1". Tianjia Bu, Hui-Chuan Eileen Wang,and Hongbin Li. Langmuir, 28,12319-12325, (2012).

"Engineered Bi-Histidine Metal Chelation Sites Map the Structure of the Mechanical Unfolding Transition State of an Elastomeric Protein Domain GB1". Tao Shen, Yi Cao, Shulin Zhuang, and Hongbin Li. Biophysical Journal, 103, 807-816, (2012).

"A Facile Way to Tune Mechanical Properties of Artificial Elastomeric Proteins-Based Hydrogels". Jie Fang and Hongbin Li. Langmuir, 28, 8260-8265, (2012).

"Mechanically Untying a Protein Slipknot: Multiple Pathways Revealed by Force Spectroscopy and Steered Molecula Dynamics Simulations". Chengzhi He, Georgi Z. Genchev, Hui Lu, and Hongbin Li. JACS, 134, 10428-10435, (2012).

"Single Molecule Force Spectroscopy Reveals Critical Roles of Hydrophobic Core Packing in Determining the Mechanical Stability of Protein GB1". Tianjia Bu, Hui-Chuan Eileen Wang, and Hongbin Li. Langmuir, 28, 12319-12325, ( 2012).

"Hydrogen Bond Strength Modulates the Mechanical Strength of Ferric-Thiolate Bonds in Rubredoxin". Peng Zheng,Shin-ichi J. Takayama, A. Grant Mauk, and Hongbin Li.Journal of the American Chemical Society, 134(9), 4124−4131, (2012).

"Tandem Modular Protein-Based Hydrogels Constructed Using a Novel Two-Component Approach". Shanshan Lv, Yi Cao and Hongbin Li.Langmuir, 28(4),2269-2274,(2011).

"Dynamics of Protein Folding and Cofactor Binding Monitored by Single-Molecule Force Spectroscopy". Yi Cao and Hongbin Li. Biophysical Journal, 101(8),2009-2017, (2011)

"Enhancing the Mechanical Stability of Proteins through a Cocktail Approach". Yi Cao, Yongnan Devin Li,Hongbin Li. Biophysical Journal, 100(7), 1794-1799,(2011).

"Direct Measurements of the Mechanical Stability of Zinc-Thiolate Bonds in Rubredoxin by Single-Molecule Atomic Force Microscopy". Peng Zheng and Hongbin Li.Biophysical Journal 101,1467-1473,(2011).

"Kinetic Partitioning mechanism Governs the Folding of the Third Fnll Domain of Tenascin-C: Evidence at the Single-Molecule level". Qing Peng, Jie Fang, Meijia Wang, Hongbin Li.Journal of Molecular Biology, 412(4), 698-709,(2011).

"Single-Molecule-Level Evidence for the Osmophobic Effect". Daniel Aioanei, Shanshan Lv,Isabella Tessari, Aldo Rampioni, Luigi Bubacco,Hongbin Li, Bruno Samorì, Marco Brucale.Angewandte Chemie.123(19), 4486-4489,(2011).

" Facile Method of Constructing Polyproteins for Single-Molecule Force Spectroscopy Studies". Peng Zheng, Yi Cao, Li Hongbin.Langmuir 27(10), 5713-8 (2011).

"Highly Covalent Ferric-thiolate Bonds Exhibit Surprisngly Low Mechanical Stability". Peng Zheng, Li Hongbin. Journal of the American Chemical Society, 133(17),6791-8, (2011).

"Single Molecule Force Spectroscopy Reveals that Electrostatic Interactions Affect the Mechanical Stability of Proteins", Peng Zheng, Yi Cao, Tianjia Bu, Suzana K. Straus and Hongbin Li. Biophysical Journal 100(6), 1534-41,(2011).

"Single-Molecule Force-Clamp Spectroscopy: Dwell Time Analysis and Practical Considerations", Yi Cao and Hongbin, Langmuir, 27(4), 1440-1447 (2011).

"Phenotypic effects of Ehlers-Danlos syndrome-associated mutation on the Fnll domain of tenascin-X", Shulin Zhuang, Api Linhananta and Hongbin Li, Protein Sciences 19, 2231-2239 (2010).

"Measuring "unmeasurable" folding kinetics of proteins by single-molecule force spectroscopy." Ashlee Jollymore and Hongbin Li, Journal of Molecula Biology 402, 610-617 (2010).

"Protein mechanics: from single molecules to functional biomaterials." Hongbin Li and Yi Cao, Accounts of Chemical Research 43, 1331-1341(2010).

"Designed biomaterials to mimic the mechanical properties of muscles". Shanshan Lv, Daniel M.Dudek, Yi Cao, M.M. Balamurali, John Gosline & Hongbin Li. Nature.465, 69-73 (2010).

"Domain insertion effectively regulates the mechanical unfolding hierarchy of elastomeric proteins: towards engineering multi-functional elastomeric proteins". Peng Q, Li H. Journal of the American Chemical Society. 131(39):14050-6. (2009)

"Direct observation of the tug-of-war during the folding of a mutually exclusive protein". Peng Q, Li H. Journal of the American Chemical Society. 131(37):13347-54. (2009)

"Modulating the mechanical stability of extracellular matrix protein tenascin-C in a controlled and reversible fashion". Zhuang S, Peng Q, Cao Y, Li H. Journal of Molecular Biology. 390.820-829. (2009).

"A force-spectroscopy-based single-molecule metal-binding assay", Cao Y, Er KS, Parhar, Li H. ChemPhysChem. 10. 1450-1454(2009).

"Mechanical Design of the Third FnIII Domain of Tenascin-C", Qing Peng, Shulin Zhuang, Meijia Wang, Yi Cao, Yuanai Khor, and Hongbin Li. Journal of Molecular Biology. 386. 1327-1342 (2009).

"Nanomechanical Properties of Tenascin-X Revealed by Single Molecule Force Spectroscopy", Ashlee Jollymore, Claire Lethias, Qing Peng, Yi Cao and Hongbin Li*, Journal of Molecular Biology, 385,1277-1286 (2009).

" Recombination of protein fragments: a promising approach toward engineering proteins with novel nanomechanical properties". Balamurali MM, Sharma D, Chang A, Khor D, Chu R, and Hongbin Li* Protein Sci. 17, 1815-26 (2008).

"Stabilization provided by neighboring strands is critical for the mechanical stability of proteins", Sharma D, Feng G, Khor D, Genchev GZ, Lu H, and HongbinLi*, Biophysical Journal, 95, 3935-42 (2008).

""Mechanical Engineering" of Elastomeric Proteins: Toward Designing New Protein Building Blocks for Biomaterials (Feature Article)", Hongbin Li*, Advanced Functional Materials 18 , 2643-2657 (2008).

"Single molecule force spectroscopy reveals engineered metal chelation is a general approach to enhance mechanical stability of proteins" Yi Cao, Teri Yoo, and Hongbin Li* Proceedings of the National Academy of Sciences USA, 105, 11152-11157 (2008).

"Engineering tandem modular protein based reversible hydrogels" Yi Cao and Hongbin Li*, Chemical Communications,35, 4144–4146 (2008).

" Engineered elastomeric proteins with dual elasticity can be controlled by a molecular regulator" Yi Cao and Hongbin Li*, Nature Nanotechnology, 3, 512-516 (2008).
(This paper has been highlighted by Nanowerk and news of UBC SCIENCE.)

"Configuration Entropy Modulates the Mechanical Stability of Protein GB1", Hongbin Li*, Hui-Chuan Wang, Yi Cao, Deepak Sharma, Meijia Wang, Journal of Molecular Biology, 379, 871-880 (2008).

"Protein–Protein Interaction Regulates Proteins’ Mechanical Stability", Yi Cao, Teri Yoo, Shulin Zhuang, Hongbin Li*, Journal of Molecular Biology, 378, 1132-1141 (2008).

"Direct Observation of Markovian Behavior of the Mechanical Unfolding of Individual Proteins", Yi Cao, Rachel Kuske and Hongbin Li*, Biophysical Journal, 95, 782-788 (2008).

"Atomic Force Microscopy Reveals Parallel Mechanical Unfolding Pathways of T4 Lysozyme: Evidence for Kinetic Partitioning Mechanism", Qing Peng and Hongbin Li* , Proceedings of the National Academy of Sciences USA 105 , 1885-1890 (2008).

"How Do Chemical Denaturants Affect the Mechanical Folding and Unfolding of Proteins?", Yi Cao and Hongbin Li* , Journal of Molecular Biology 375 , 316-324 (2008).

"A functional single-molecule binding assay via force spectroscopy", Yi Cao, M. M. Balamurali, Deepak Sharma, and Hongbin Li* , Proceedings of the National Academy of Sciences USA 104 , 15677-15681 (2007).

"Engineering proteins with tailored nanomechanical properties: a single molecule approach (INVITED REVIEW ARTICLE)", Hongbin Li* , Organic and Biomolecular Chemistry 5 , 3399-3406 (2007).

"Polyprotein of GB1 is an ideal artificial elastomeric protein", Cao, Y. and Li, H. B. , Nature Materials 6 , 109-114 (2007).

"Engineering Proteins with Novel Mechanical Properties by Recombination of Protein Fragments", Sharma, D., Cao, Y. and Li, H. B. , Angewandte Chemie International Edition 45 1-7 (2006).
(It has been selected by Angewandte Chemie as a "hot paper")
(It has been highlighted by Angewandte Chemie's Press Release)
(also see press release from Sciencedaily).

"Single Molecule Force Spectroscopy Reveals a Weakly Populated Microstate of the FnIII Domains of Tenascin", Cao, Y. and Li, H. B. , Journal of Molecular Biology 361 , 372-381 (2006).

"Nonmechanical Protein Can Have Significant Mechanical Stability", Yi Cao, Canaan Lam, Meijia Wang, and Hongbin Li* , Angew. Chem. Int. Ed. 45 , 642-645 (2006).

"The Unfolding and Folding Dynamics of TNfnALL Probed by Single Molecule Force-ramp Spectroscopy", Meijia Wang, Yi Cao and Hongbin Li* , Polymer 47 2548-2554 (2006).

"Force-Clamp Spectroscopy Monitors the Folding Trajectory of a Single Protein ", Fernandez, J. M. and Li, H. B. , Science 303 , 1674-1678 (2004).

"The Force-Driven Conformations of Heparin Studied with Single Molecule Force Microscopy", Marszalek, P. E.; Oberhauser, A. F.; Li, H. B.; Fernandez, J. M. , Biophysical Journal 85 , 2696-2704 (2003).

"The mechanical stability of ubiquitin is linkage dependent", Carrion-Vazquez, M.; Li, H. B.; Lu, H.; Marszalek, P. E.; Oberhauser, A. F.; Fernandez, J. M. , Nature Structural Biology 10 , 738-743 (2003).

"Mechanical design of the first proximal Ig domian of human cardiac titin revealed by single molecule force spectroscopy", Li, H. B. and Fernandez, J. M. , Journal of Molecular Biology 334 , 75-86 (2003).

"PEVK domain of titin: an entropic spring with actin-binding properties", Linke, W. A.; Kulke, M.; Li, H. B.; Fujita-Becker, S.; Neagoe, C.; Manstein, D. J.; Gautel, M.; Fernandez, J. M. , Journal of Structural Biology 137 , 194-205 (2002).

"Reverse engineering of the giant muscle protein titin", Li, H. B.; Linke, W. A.; Oberhauser, A. F.; Carrion-Vazquez, M.; Kerkvliet, J. G.; Lu, H.; Marszalek, P. E.; Fernandez, J. M. , Nature 418 , 998-1002 (2002).

"Fingerprinting polysaccharides with single molecule atomic force microscopy", Marszalek, P. E.; Li, H. B.; Fernandez, J. M. , Nature Biotechnology 19 , 258-262 (2001).

"Multiple conformations of PEVK proteins detected by single molecule techniques", Li, H. B.; Oberhauser, A. F., Redick, S. D., Carrion-Vazquez, M.; Erickson, H. P.; Fernandez, J. M. , Proceedings of the National Academy of Sciences USA 98 , 10682-10686 (2001).

"AFM captures quantized plastic deformation in gold nanowires", Marszalek, P. E.; V. J. Greenleaf; Li, H. B.; Oberhauser, A. F.; Fernandez, J. M. , Proceedings of the National Academy of Sciences USA 97 , 6282-6286 (2000).

"Hydrogen bonding governs the elastic properties of poly(vinyl alcohol) in water: single-molecular force spectroscopic studies on PVA", Li, H. B.; Zhang, W. K.; Xu, W. Q.; Zhang, X. , Macromolecules 33 , 465-469 (2000).

"Atomic force microscopy reveals the mechanical design of a modular protein", Li, H. B.; Oberhauser, A. F.; Fowler, S.; Clarke, J.; Fernandez, J. M. , Proceedings of the National Academy of Sciences USA 97 , 6527-6531 (2000).

"Point mutations alter the mechanical properties of immunoglobulin modules", Li, H. B.; Carrion, M.; Oberhauser, A. F., Marszalek, P. E.; Fernandez, J. M. , Nature Structural Biology 7 , 1117-1120 (2000).

"Force Spectroscopy on Single Xanthan Molecules", Li, H. B.; Rief, M.; Oesterhelt, F.; Gaub, H. , Applied Physics A-Materials Science & Processing 68 , 407-410 (1999).

"Atomic levers control pyranose ring conformations", Marszalek, P. E.; Pang, Y. P.; Li, H. B.; Oberhauser, A.; Fernandez, J. M. , Proceedings of the National Academy of Sciences USA 96 , 7894-7898 (1999)

"Single Molecule Force Spectroscopy on Polyacrylic Acid", Li, H. B.; Liu, B.; Zhang, X.; Gao, C.; Shen, J.; Zou, G , Langmuir 15 , 2120-2124 (1999).

"Single Molecule Force Spectroscopy on 1,4-linked Polysaccharides", Li, H. B.; Rief, M.; Oesterhelt, F.; Gaub, H.; Zhang, X.; Shen, J. , Chemical Physics Letters 305 , 197-201 (1999).

"Mechanical unfolding intermediates in titin modules", Marszalek, P. E.; Lu, H.; Li, H. B.; Carrion-Vazquez, M.; Oberhauser, A. F.; Fernandez , Nature 402 , 100-103 (1999).

"Single Molecule Force Spectroscopy on Polyvinyl Alcohol", Li, H. B.; Zhang, W.; Zhang, X.; Shen, J.; Liu, B.; Gao, C., Zou, G. , Macromolecular Rapid Communication 19 , 609-611 (1998).

"Well Ordered Structure and Phase Transition Behaviors of a Reversed Duckweed Polymeric LB Film Studied by FTIR and SAXD", Li, H. B.; Wang, Z.; Zhao, B.; Xiong, H.; Zhang, X.; Shen, J.C. , Langmuir 14 , 423-428 (1998).

"Single Molecule Force Spectroscopy on Xanthan by AFM", Li, H. B.; Rief, M.; Oesterhelt, F.; Gaub, H. , Advanced Materials 10 , 316-319 (1998).

"Thermotropic Liquid Crystals Behaviors of an Amphiphilic Polymer not Bearing Mesogens", Li, H. B.; Zhang, X.; Zhang, R.; Shen, J.; Zhao, B.; Xu, W. , Macromolecule 28 , 8178-8181 (1995).